Journal: bioRxiv

Article Title: KRAB zinc finger proteins ZNF587/ZNF417 protect lymphoma cells from replicative stress-induced inflammation

doi: 10.1101/2023.03.08.531722

Figure Lengend Snippet: (a) Quantitative RT-PCR analysis of ZNF587B, ZNF814, ZNF587, ZNF417, and ZNF586 mRNAs 5 days after LV transduction with shRNAs targeting ZNF586, ZNF587B/ZNF814, and ZNF587/ZNF417 paralog pairs. Statistics: Student’s t-test. (b) Top: Bar plots showing the relative expression of ZNF586, ZNF417, ZNF587, ZNF587B, and ZNF814 in U2932 compared to OCI-Ly7 using GUSB, TBP, and/or ALAS2 housekeeping genes for normalization. Bottom: Bar plots showing the relative expression of ZNF587 and ZNF417 in SUDHL4, U2932, HBL1 cells compared to OCI-Ly7 using GUSB, TBP, and/or ALAS2 housekeeping genes for normalization. (c) MTT proliferation assays of U2932 and OCI-Ly7 upon LV transduction with two different anti-ZNF587/417 shRNAs, or control shRNA (shScr). Cells were plated after 5 days of LV transduction and 3 days of puromycin selection. (d) MTT proliferation assay of human primary dermal fibroblasts upon LV transduction with two anti-ZNF587/417 shRNAs or control shRNA. Cells were plated after 5 days of LV transduction and 3 days of puromycin selection. Quantitative RT-PCR analysis of ZNF587 and ZNF417 mRNAs 5 days after LV transduction. Statistics: Student’s t-test. (e) Scatter plot of RNA-seq from ZNF417/587 U2932 KD versus shScr control cells at day 3 of KD, outlining DEGs (grey dots, FDR <0.05) and among them genes belonging to the UV response UP Hallmark gene set (dark red dots) and MHC Class I genes (blue dots).

Article Snippet: Primary normal dermal fibroblasts were cultured in Fibroblast Basal Medium (ATCC) supplemented with Fibroblast Growth Kit-Low Serum (ATCC).

Techniques: Quantitative RT-PCR, Transduction, Expressing, Control, shRNA, Selection, Proliferation Assay, RNA Sequencing

Journal: Cardiovascular Diabetology

Article Title: RhoA/ROCK-dependent moesin phosphorylation regulates AGE-induced endothelial cellular response

doi: 10.1186/1475-2840-11-7

Figure Lengend Snippet: AGE-HSA induced time-(A) and dose-(B) dependent increases of HMVEC monolayer permeability . HMVECs were treated with 50 mg/L AGE-HSA for 1, 2, 4, 8, or 12 h, or with 12.5, 25, 50, or 100 mg/L AGE-HSA for 8 h. Culture medium was used as the blank control and HSA was used as the albumin control. Permeability was measured from the transflux of tracer protein TRITC-albumin across monolayers and was expressed as a coefficient for albumin (Pa). n = 3, * P < 0.05, ** P < 0.01 vs control.

Article Snippet: A human dermal microvascular endothelial cell (HMVEC) line was purchased from Cell Applications (San Diego, CA) [ ].

Techniques: Permeability, Control